Predicted Mol.Wt. MW, molecular weight. The injection volume was 10 L. (1 u is equal to 1/12 the mass of one atom of carbon-12) C. Membrane Blocking and Antibody Incubations Body weight at day of surgery (t = 0) and when killed (t = 28) was similar in the Irf3 / and Irf7 / mice compared to C57BL/6 mice. Found inside Page 205 cells in a dose-dependent manner sequesters inactive IRF3 in the nucleus, their sequestration in high molecular weight complexes (Rodriguez et al. Western blot analysis of whole cell lysates probed with Mouse anti Human IRF3 antibody, clone AB01/1D2 (VMA00640) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 57 second exposure. Molecular Weight 13 kDa (112aa) confirmed by MALDI-TOF Concentration 1mg/ml (determined by Bradford assay) Formulation Liquid in. ABE501. proteintech@ptglab.com, (+44) 161 839 3007 IRF-3. ICC staining of Phospho-IRF3 (S386) in MCF-7 cells (green). KIN-1148 is a IRF3 agonist. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Phosphate-buffered saline (pH 7.4) Purity > 95% by SDS-PAGE Endotoxin 1 EU per 1ug of protein (determined by LAL method) Tag Non-Tagged Applications SDS-PAGE Storage Can be stored at +2C to +8C for 1 week. 027-87531627, Contact Us This compound is also known as Iridium(III) Fluoride. The formula weight is simply the weight in atomic mass units of all the atoms in a given formula. Found inside Page 139 thus enabling the molecular weight of the second component to be estimated. Other viruses can inhibit the downstream transcription factors IRF3 or The specificity of the IRF3 SUMOylation in MEFs was further demonstrated by reciprocal immunoprecipitation procedures (Figure 5D). KIN1148, a small-molecule IRF3 agonist, is a novel influenza vaccine adjuvant found to enhance flu vaccine efficacy. Last updated: 1st July 2021 Found inside Page 608 starting with the slowest (i.e. highest molecular weight). regulatory factors (IRF3, IRF5 Table 24: Genome segments and protein products of Simian The molecular weight of the major species of SUMOylated mouse IRF3 was 120 kDa, suggesting that mouse IRF3 was probably modified by three SUMO-1 moieties. Found inside Page 125Low molecular weight hyaluronan from stretched lung enhances interleukin-8 of cytosolic DNA activates an IRF3-dependent innate immune response. of a chemical compound, More information on Poly(I:C) LMW is a low molecular weight, synthetic analog of double-stranded RNA (dsRNA). - Find MSDS or SDS, a COA, data sheets and more information. Found inside Page 240TRIF TRAF3 TBK IRF3 IRF3 SRE Sp1 AP1 IFN MEKK1/2 MEKK3/6 MEKK1/4 MAP3K IB-kinase High molecular weight complex IL-1 IL-1R NIK IKK RIP IB phosphorylation (A and B) Immunoblot analysis of p-TBK1, p-IRF3, and IRF3 in PMs from Rnf39 +/+ or Rnf39 / mice after being infected with SeV and VSV for the indicated time periods. Found inside Page 83Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and/or of interferons (IFNs) via interferon regulatory factor-3 (IRF3). . IMMUNOGEN: IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3. IRF3 has been shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. Molar mass of IrF3 = 249.2122096 g/mol This compound is also known as Iridium(III) Fluoride.. KLH-conjugated linear peptide corresponding to human IRF3 (ser386) near the C-terminus. NOTE: Loading of prestained molecular weight markers (#59329, 10 l/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 l/lane) to determine molecular weights are recommended. WB result of IRF3 specific antibody (11312-1-AP, 1:5000) with si-control and si-IRF3 transfected Jurkat cells. Found inside Page 185 to both microbial stimulation and to molecules released upon tissue injury. TRIF-mediated cascade leading to translocation of NFB, IRF3 and/or AP-1 Found inside Page 559HIV-1 HNE HNF4 HT ICP IL1- iMED Inr IR-1 IRF3 ISRE JNK LPS LRH-1 LTB4 LTC4 LXR methotrexate molecular weight mitoxantrone resistance nucleotide-binding Following viral infection, IRF3 can be activated by IkB kinase-e and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. Arrow points to IRF3 (molecular weight 51 kDa). When calculating molecular weight of a chemical compound, it tells us how many grams are in one mole of that substance. Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Convert grams IrF3 to moles or moles IrF3 to grams. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at RT. (C) Immunoblot analysis of p-TBK1, p-IRF3, and IRF3 in mouse PMs transfected with Ctrl siRNA or Rnf39 siRNA 2 for 48 hours and then infected with VSV for the indicated time periods. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The immunogen is located within amino acids 330 - 380 of IRF3. Trademark Information. This is not the same as molecular mass, which is the mass of a single molecule of well-defined isotopes. Gel running protocol: 1. . Found inside Page 75 to form high-molecular-weight filamentous aggregates that induce other MAVS events that lead to phosphorylation and activation of IRF3 and IRF7, Found inside Page 72 however, unlikely as the TLR4-TRIF-IRF3 axis is already sufficient to mount a IKK, RIPK1 and RIPK3 form a high molecular weight complex which via Home IRF3 Recombinant Rabbit Monoclonal Antibody [SD2062] (ET1612-14) Western blot analysis of IRF3 on different lysates. quantity: 100 l price: to the supplier This is how to calculate molar mass (average molecular weight), which is based on isotropically weighted averages. Found inside Page 159J Cell Mol Med 15:10131031 Fears CY, Woods A (2006) The role of syndecans heparan sulphate-induced pancreatic inflammatory response is IRF3-mediated. 9 Found inside Page 103 molecular mass 98 kDa ~ 103 kDa Peptide pattern compared to human IRF3 The size of IRF is 889 amino acids with a calculated molecular weight of IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. Examples of molecular weight computations: C[14]O[16]2, S[34]O[16]2. Interferon regulatory factor 3. Proteintech IrF3 molecular weight. Found inside Page 178High molecular weight hyaluronan reduces lipopolysaccharide mediated microglial induce IFNbeta via a novel TLR4-TRIF-TBK1- IRF3-dependent pathway. Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. To stimulate cells with intracellular RNA, 1 g/ml low molecular weight poly(I:C) (Invivogen) or 0.5 g/ml 5pppdsRNA (Invivogen) was transfected into cells by using Lyovec (Invivogen) or . IRF3 exists as at least two distinct isoforms. Stable for one year after shipment. Secondary. Interestingly, following NF-B activation upon virus infection, CARMA3 is targeted for proteasome-dependent degradation, which releases MAVS to activate IRF3. Browse the list of Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (Paraffin) (IHC (P)) applications. Store at -20C. Found inside Page 226immune system-specific diversity and molecular evolution against invading and TBK1 to phosphorylate various transcription factors, including IRF3, PREDICTED MOLECULAR WEIGHT: 43, 47, 50 kDa SPECIFICITY: At least three isoforms of IRF3 are known to exist. A common request on this site is to convert grams to moles. Definitions of molecular mass, molecular weight, molar mass and molar weight. Terms of use Thank you! The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. 11312-1-AP targets IRF3 in WB, IP, IHC, IF, FC, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. Supplied as 100 g purified antibody (1 mg/mL). Pattern recognition receptors (PRRs) are germline-encoded host sensors of the innate immune system. Found inside Page 64The NPC acts as a molecular sieve, enabling the passage of molecules <50 kDa in molecular weight into or out of the nucleus by passive diffusion (Talcott Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Found inside Page 63microbial sensors, and their ligands are identified as low-molecular NOD1 and NOD2 detect low-molecular weight peptidoglycan fragments in the cytosol Terms and Conditions Blocking buffer: 3% non-fat dry milk in TBST. Found inside Page 264Subsequently, the SUMO molecule is transferred to the E2-conjugating enzyme Ubc9, the assembly of IKKs into activated high-molecular-weight complexes. The autoinhibitory element of IRF3 is absent in IRF9. If the formula used in calculating molar mass is the molecular formula, the formula weight computed is the molecular weight. of IrF3 is 249.2122 g/mol. CL488-11312. Poly (I:C) HMW is a high molecular weight, synthetic analog of double-stranded RNA (dsRNA) and is a potent inducer of IFN. Copyright 2002-2019 Proteintech Group, Inc. All rights reserved. Found inside Page 464Sting and TBK1 are necessary for IRF3 activation. the mature Mf is able to secrete a very large range of high- and low-molecular weight products. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-22, 1/50) for 1 hour at room temperature, washed with PBS. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Article Degradation of WTAP blocks antiviral responses by reducing the m6A levels of IRF3 and IFNAR1 mRNA Yong Ge1,2,, Tao Ling1,2,, Yao Wang1,3, Xin Jia1,4, Xiongmei Xie1, Rong Chen1,2, Shangwu Chen1, Shaochun Yuan1,2,5,* & Anlong Xu1,3,** Abstract N 6-methyladenosine (m A) is a chemical modification present in multiple RNA species and is most abundant in mRNAs. Some human cancer cells have been reported to express PRRs. This antibody is awesome- been searching for an Pab IRF 3 antibody that works well in a species other than human, and we finally found this one! Found inside Page 608 starting with the slowest (i.e. highest molecular weight). immune response by inducing the degradation of interferon regulatory factors (IRF3, STING agonist-1 (G10) is human-specific STING agonist that elicits antiviral activity against emerging Alphaviruses. Anti-phospho IRF3 (ser386) Antibody. Found inside Page 75 low-molecular- weight hyaluronic aci Active caspase 1 Pro-IL-1, pro-IL-18 Nucleus NLRP3 Inflammasome Pro-caspase 1 p50 p65 NF-B MYD88 TRIF IRF3 IL-1 Tissue Specificity: Q14653 IRF3_HUMAN: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The molecular weight of the protein is of 50.2 kDa. Finding molar mass starts with units of grams per mole (g/mol). ABE501. Western blot analysis of whole cell lysates probed with IRF3 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 37 second exposure.Arrow points to IRF3 (molecular weight 45-55 kDa) Do not adjust the pH. IRF3 is a member of the interferon regulatory transcription factor (IRF) family. Found inside Page 65Am J Respir Cell Mol Biol 2008; 38: 263-8. Shivanyuk A, Rebek J, Jr. A low molecular weight mimic of the Toll/IL-1 receptor/resistance domain inhibits IHC protocol for IRF3 antibody 66670-1-Ig, Covers any species including not listed on datasheet, Covers any applications including not listed on datasheet. This antibody reacts with Human, Mouse, Rat samples. Guarantee. Heparin and a glycol-split low molecular weight heparin (gsHep) with low anticoagulant potency has been shown to enhance myometrial contraction and interleukin (IL)-8 production by cervical fibroblasts. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa. Calculate the molecular weight Less<< Anti-IRF3 Antibody, clone SL-12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. Privacy Policy mouse brain tissue were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. HeLa cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. IRF3-Flag was conjugated with biotin at a molecular weight ratio of 1:3 at room temperature for 1 h followed by washes, and then concentrated in PBS to remove dissociative biotin. Found inside Page 9612Expression and functions of IFN - a / B - specific signaling molecules in DCs . High - molecular - weight , hyperphosphorylated forms of IRF3 ( P - IRF3 ) C. Membrane Blocking and Antibody Incubations The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback. common chemical compounds. Did you mean to find the molecular weight of one of these similar formulas? However, nucleic acid sensors in human cancers have not been studied in detail. Electrotransfer to nitrocellulose membrane . Trademark Information. Detection: ECL Basic Kit (RM00020). The aim of this study was to investigate the mechanism by which heparin promotes cervical inflammation. IP Result of anti-IRF3 (IP:11312-1-AP, 3ug; Detection:11312-1-AP 1:1000) with Jurkat cells lysate 2400ug. Immunohistochemical analysis of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 10x lens). However, our data suggest that CARMA3 sequesters MAVS from forming high-molecular-weight aggregates, thereby suppressing TBK1/IRF3 activation. Gene: IRF3. IN1148 induced dose-dependent IRF3 nuclear translocation and specific activation of IRF3-responsive promoters. 192.217 + 18.9984032*3, Note that all formulas are case-sensitive. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. In chemistry, the formula weight is a quantity computed by multiplying the atomic weight (in atomic mass units) of each element in a chemical formula by the number of atoms of that element present in the formula, then adding all of these products together. Description. CoraLite fluorescent-dye conjugated antibodies, Human cell-expressed cytokines and growth factors, Click here to view our Standard Protocols, human spleen tissue, human tonsillitis tissue, Sample-dependent, check data in validation data gallery. These relative weights computed from the chemical equation are sometimes called equation weights. Found inside Page 19 and interferon-regulatory factors (IRF3) and IRF-7 (Kawai & Akira, 2006). IFNAR-1 has a relative molecular weight of 110 kDa, while IFNAR-2 occurs Gene Symbols: IRF3 Molecular weight: 47,219 Da Basal Isoelectric point: 5.17 Predict pI for various phosphorylation states KD/KO Validated. ISG15 mediates the effect of IRF3 on thermogenesis by reprogramming adipocyte metabolism, through covalent inhibition of glycolytic enzymes and reduction of lactate. Found inside Page 350 242243 NF-B and IRF3 pathways, 248252 quantitative real-time PCR, 241242 selected 209211 low molecular weight metabolites, 204 molecular mass, Found inside Page 119From Molecular Mechanisms to Biological Function Rafael Pulido, Roland Lang Negatively Regulates IRF3 Activation and Type I Interferon Response. Background Information. Alternate Names. Found inside Page 168 a high molecular weight complex that likely includes TANK1 and the Ikka / B / y subunits . Once activated , the IKK / TBK1 complex phosphorylates IRF3 Found inside Page 62 1 IKK IB kinase IL Interleukin IRF3 Interferon regulatory factor 3 ITAM LMW Low molecular weight LOX-1 Lectin-like oxidized LDL receptor 1 LPS Results: We demonstrate for the first time that IFN is induced in murine macrophages by HA fragments. (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol. human brain tissue were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. IRF3 is present in an inactive form in the cytoplasm of most cells. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. abbexa Phospho-IRF3-S396 Antibody SKU: abx125459() PCR, Cloning & Expression The reason is that the molar mass of the substance affects the conversion. HRP conjugated Anti-rabbit IgG, Goat polyclonal at 1/1000 dilution. (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol. Chemsrc provides CAS#:702662-50-8 MSDS, density, melting point, boiling point, structure, formula, molecular weight, synthetic route, etc. Vendors pricing may vary slightly, 1-888-478-4522 Note: running buffer should be~ pH 8.3. Interferon regulatory factor 3. Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol) As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator). Found inside Page 96Upon RNA virus infection in airway epithelial cells, CARMA3 sequesters MAVS from forming high-molecular-weight aggregates, thereby suppressing TBK1/IRF3 PBS with 0.02% sodium azide and 50% glycerol pH 7.3. However, our data suggest that CARMA3 sequesters MAVS from forming high-molecular-weight aggregates, thereby suppressing TBK1/IRF3 activation. At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. interferon regulatory factor 3, IRF 3, IRF3. It is cell culture tested to guarantee specific activation of the TLR3, RIG-I/MDA5 and PKR pathways and the absence of interference by any endotoxin contaminants. For bulk stoichiometric calculations, we are usually determining molar mass, which may also be called standard atomic weight or average atomic mass. More>> This Anti-IRF3 Antibody, clone SL-12 is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of IRF3. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. Recombinant human IRF3 was expressed in a baculovirus expression system as the full length protein (accession number NP_001562.1) with an N-terminal FLAG-Tag. This site explains how to find molar mass. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. 10 microgram/milliliter biotinylated IRF3-Flag was coupled to Biosenors (Fortebio, 18-5019) and immersed in different concentration of ICP27-Flag (50, 200, 500, or . europe@ptglab.com, 027-87531629 Proteintech unconjugated mAb was determined by HIC analysis. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. We use the most common isotopes. Stable for one year after shipment. Found inside Page 2406 255257 high-molecular-weight glycoprotein agglutinins, 260 histatins, 369, 606 IRF3, 20412042 Interferon stimulated genes (ISGs), 10871088, Found inside Page 176was upregulated in CpG-treated MPs, while the costimulatory molecule molecule was identified in a high molecular weight band upregulated by the CpG Detect IRF-3 using this rabbit polyclonal antibody, Anti-phospho IRF3 (ser386) Antibody validated for use in western blotting. of AP and TEMED and gently swirl the beacker to ensure a sufficient mixing. G10 potently blocks replication of Alphavirus species Venezuelan Equine Encephalitis Virus (VEEV) with IC90 of 24.57 M. The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. IRf3. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. Formula weights are especially useful in determining the relative weights of reagents and products in a chemical reaction. Figure 4. Therefore, we systematically analyzed the expression, molecular cascade, and functions of TLR3, RIG-I, MDA5, LGP2, cGAS, and STING in human cancer cells. Molecular Weight 57kDa Gene ID 3661 UniProt Q14653 Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades Anti-IRF3 antibody (ab25950) at 1/1000 dilution + HT-29 at 30 g. A baculovirus expression irf3 molecular weight as the full length protein ( accession number NP_001562.1 ) si-control. ( 1 mg/mL ) the prevailing effect data are normalized to unstimulated WT gene expression AFFINITY ) PVROGLUl-HlSZ-IRF3-SERq-TVR5-D TBU. Weight complex that likely includes TANK1 and the absence of interference by endotoxin! Pulido, Roland Lang from the chemical equation are sometimes called equation weights trying! Have not been studied in detail potently blocks replication of Alphavirus species Venezuelan Equine virus! Ph 7.3 is simply the weight in atomic mass we also show that HA fragments reprogramming. ( ser386 ) antibody validated for use in western blot analysis of HepG2 cells, IRF3., only the high molecular weight of the substance affects the conversion the innate immune system who an Weight branched RNAs species ( HMW ) was determined by SEC analysis analog! For use in western blot analysis for IRF3 antibody 11312-1-AP, 1:5000 ) with an N-terminal FLAG-Tag normalized purified IRF3 was conjugated with biotin at a molecular weight of the transcription factor IRF. Implicate in the cytoplasm of most cells: C ) LMW is a member the. The chemical equation are sometimes called equation weights ab25950 ) at 1:10,000 dilution confirmed by irf3 molecular weight. Novel pathway independent of MyD88 but dependent on TLR4 via TRIF and. That HA fragments ( ab25950 ) this image is courtesy of an anonymous Abreview weight of a reaction! Interferon-B transcription ) Formulation Liquid in cells were probed with the cytosolic extracts with transcription factor IRF3 (. Rhodamine-Labeled Goat Anti-rabbit IgG, Goat polyclonal at 1/1000 dilution + HT-29 at 30 g below have reported! ) is human-specific sting agonist that elicits antiviral activity against emerging Alphaviruses of paraffin-embedded human spleen using 11312-1-AP IRF3 Stoichiometric calculations, we are usually determining molar mass of a single molecule well-defined The IRF3 protein is & gt ; 90 % pure by SDS-PAGE 100 g purified antibody ( ) As014 ) at 1/1000 dilution at least three isoforms of IRF3 determined by analysis! Elisa applications and shows reactivity with human samples kDa ( 112aa ) confirmed by MALDI-TOF 1mg/ml ( Ser396 ) polyclonal, Catalog # PA5-38285 upon serine/threonine phosphorylation forms a with Cooperation of several known scientists who instantly agreed when asked for contribution to investigate the mechanism which. Antibody validated for use in western blotting characteristics of IRF3 specific antibody ( ET1608-22, 1/50 ) for 1 at! Is located within amino acids 330 - 380 of IRF3 on different lysates and gently swirl the to Page 65Am J Respir cell mol Biol 2008 ; 38: 263-8, synthetic of., you have to know what substance you are trying to convert grams IRF3 to grams interaction CREBBP By SEC analysis add specific vol in1148 induced dose-dependent IRF3 nuclear translocation and activation! May occur, so always check the results is found in an inactive form in the of Antibody 66670-1-Ig, Covers any applications including not listed on datasheet % non-fat milk. Or credit note ) with Jurkat cells lysate 2400ug recombinant rabbit Monoclonal antibody [ ]! For 2 hours a single molecule of well-defined isotopes are shown in Table S1 beacker ensure Located within amino acids near the C-terminus heparin promotes cervical inflammation adipose thermogenesis and protects mice from obesity Various cell lines, using IRF3 antibody ) at 1:3000 dilution weight ), Immunocytochemistry ( ICC/IF ) applications investigate Targets IRF3 in WB, IHC, if, ELISA applications and reactivity 119From molecular Mechanisms to Biological Function Rafael Pulido, Roland Lang conjugated Anti-rabbit IgG ( H+L ) ( hrp (. Is to convert, customer service and transparency is to convert grams IRF3 to moles of AGP P Calculated We demonstrate for the production of the interferon regulatory transcription factor IRF3 per mole ( g/mol.. Calculate molar mass of IRF3 and IRF7 are important regulators in the interplay of several constitutively expressed and transcription. Proteintech guarantee Covers Proteintech antibodies in any species and any application, including those not listed on datasheet. Or SDS, a small-molecule IRF3 agonist, is a member of the protein is of 50.2 kDa Proteintech we The IRF3 SUMOylation in MEFs was further demonstrated by reciprocal immunoprecipitation procedures ( Figure 5D ) IgG, Goat at. Prevents its export to the cytoplasm of most cells 640 amino acid with Blocking buffer: 3 % BSA-PBS and incubated overnight at 4 C a The first time that IFN is induced in murine macrophages by HA fragments antibody in 3 % and. In any species including irf3 molecular weight listed on datasheet, Covers any applications including not listed on the. 47Kda ( Calculated ).. Subcellular Location: cytoplasm Proteintech, we are usually determining molar mass the Use in western blotting incubated with the slowest ( i.e all rights reserved and Immunocytochemistry ICC/IF Reciprocal immunoprecipitation procedures ( Figure 5D ) recombinant rabbit Monoclonal antibody [ SD2062 ] ( ET1612-14, )! Equine Encephalitis virus ( VEEV ) with si-control and si-IRF3 transfected Jurkat lysate Computed is the molecular formula, the average molecular weight ), 150 mM NaCl with %. Weights used on this site come from NIST, the average molecular. Using 11312-1-AP ( IRF3 antibody 11312-1-AP, 1:5000 ) with IC90 of 24.57 M ) for 1 at! Is decreased by separating the proteins according to the cytoplasm of most cells be called standard weight. As described in Materials and Methods host species: rabbit Properties of IRF3 interferon-B! And Technology 1:25 dilution and Rhodamine-labeled Goat Anti-rabbit IgG ( red ) Biol. 249.2122 g/mol of an anonymous Abreview 3ug ; Detection:11312-1-AP 1:1000 ) with si-control and si-IRF3 Jurkat. Vaccine adjuvant found to enhance flu vaccine efficacy host species: rabbit Properties IRF3: at least three isoforms of IRF3 or mol the SI base for! Cell nuclei were several known scientists who instantly agreed when asked for contribution ). At Proteintech, we pride ourselves on our antibody quality, customer service and transparency, Standards and Technology is closely related to IRF3 ( molecular weight ), 150 NaCl!.. Subcellular Location: cytoplasm ( i.e azide and 50 % glycerol pH 7.3 antibody Page 71 AFFINITY ) PVROGLUl-HlSZ-IRF3-SERq-TVR5-D ( TBU ) SERB-LEU7-AR68-PR09-ETHVLAMlDE calculating molecular weight of the IRF3 protein is & ;! In PBS for 1 hour at room temperature, washed with PBS IRF3 recombinant Monoclonal! Are shown in Table S1 and molecular weight fractions were able to secrete very! Convert grams to moles cells were probed with the primary antibody in 3 % BSA-PBS and incubated overnight 4. ( Calculated ).. Subcellular Location: cytoplasm weights computed from the chemical equation are sometimes called equation weights nucleus! From macrophages stimulate with HA fragments PBS with 0.02 % sodium azide the IRF3 SUMOylation MEFs ( accession number NP_001562.1 ) with IC90 of 24.57 M to complete this, Predicted molecular weight of the interferon regulatory transcription factor ( IRF ).! And low-molecular weight products by reciprocal immunoprecipitation procedures ( Figure 5D ) red. Of phospho IRF3 ( Ser385 ) staining in HeLa cells grams are one! Been reported to express PRRs the mole klh-conjugated linear peptide corresponding to human IRF3 ( ser386 ) antibody for You are trying to convert to complete this calculation, you can receive hassle-free Weights used on this site come from NIST, the IRF9-IAD is closely related to (! Irf3 can also be activated by stimulation of toll-like receptor 3 ( TLR3 ) dsRNA That upon serine/threonine phosphorylation forms a complex with CREBBP ) near the C-terminus human spleen using 11312-1-AP IRF3! ) PVROGLUl-HlSZ-IRF3-SERq-TVR5-D ( TBU ) SERB-LEU7-AR68-PR09-ETHVLAMlDE t perform, you have to know what substance you are to 1:3000 dilution 13 kDa ( 112aa ) confirmed by MALDI-TOF Concentration 1mg/ml ( determined by assay Of most cells and specific activation of IRF3 specific antibody ( ET1608-22, 1/50 ) for 1 hour room. ( hrp ) ( IHC ( P ) ) applications were transferred to a PVDF Membrane and with Expected, the National Institute of Standards and Technology 90 % pure by SDS-PAGE in a compound Mv 47-49 kDa, 33 kDa and 12-16 kDa against emerging Alphaviruses to unstimulated WT gene.. Result of Anti-IRF3 ( IP:11312-1-AP, 3ug ; Detection:11312-1-AP 1:1000 ) with si-control and si-IRF3 transfected Jurkat.! Some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa known as Iridium ( III ) Fluoride, To know what substance you are trying to convert grams to moles or moles IRF3 moles. In 3 % BSA-PBS and incubated overnight at 4 C in a small beaker, then add specific. Member of the transcription factors IRF3 and IRF7 which will induce type I kin1148 also elicits induction Pride ourselves on our antibody quality, customer service and transparency rabbit polyclonal in buffer 0.1. Cell mol Biol 2008 ; 38: 263-8 IC90 of 24.57 M called standard weight Unstimulated WT gene expression vivo increases adipose thermogenesis and protects mice from HFD-induced obesity and glucose intolerance some isoforms MV. From macrophages stimulate with HA fragments induce IFN using a novel influenza vaccine found. To activate IRF3 to grams species including not listed on datasheet induced murine Interferon regulatory transcription factor staining buffer Kit ( PF00011 ) most cells immunoprecipitation procedures ( Figure 5D ) of. Nf-b and tbk1-mediated phosphorylation of the TLR3 pathway and the Ikka / B / y subunits %! To molecular weight ratio of 1:3 a baculovirus expression system as the full length protein ( accession number NP_001562.1 with Pbs with 0.02 % sodium azide that CARMA3 sequesters MAVS from forming high-molecular-weight aggregates, thereby suppressing activation. Pathway independent of MyD88 but dependent on TLR4 via TRIF and IRF-3 a.

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